Skip to main content
978-487-1100

Breaking Down mRNA Capping Efficiency with High-Resolution IP-RP LC

Evaluating 5′ capping efficiency is a critical quality attribute in therapeutic mRNA manufacturing, directly impacting translation efficiency and transcript stability. However, separating structurally similar capped, partially capped, and uncapped species differing by just a single nucleotide remains a significant chromatographic challenge.

Our latest Application Note highlights an optimized ion-pair reversed-phase liquid chromatography (IP-RP LC) method designed to tackle this complexity.

𝗔𝗱𝘃𝗮𝗻𝗰𝗲𝗱 𝗕𝗶𝗼𝗶𝗻𝗲𝗿𝘁 𝗛𝗮𝗿𝗱𝘄𝗮𝗿𝗲: Utilizing the widepore YMC Accura Triart Bio C18 column to eliminate unwanted secondary interactions and deliver robust, reproducible results.

𝗦𝗲𝗮𝗺𝗹𝗲𝘀𝘀 𝗟𝗖-𝗠𝗦 𝗜𝗻𝘁𝗲𝗴𝗿𝗮𝘁𝗶𝗼𝗻: Successfully applied to LC-MS workflows using mass-extracted ion chromatograms (MICs) to definitively differentiate between Cap-0, Cap-1, and critical uncapped impurities.

Read the complete application data  or reach out to our team to learn how to optimize your oligonucleotide and mRNA separations!

Posted on May 21, 2026.

Related Posts

September 21, 2026

Join us for our upcoming webinar titled Peptide Purification: From HPLC Origins to Process Intensification

This presentation explores the evolution of peptide purification, from the early days of reversed-phase HPLC to today’s large-scale manufacturing challenges. It covers practical strategies for preparative method development, stationary phase selection, and efficient scale-up, illustrated by a case study on tirzepatide purification. The session also introduces continuous chromatography using MCSGP technology and highlights its potential for process intensification in modern peptide manufacturing.
Read More

September 4, 2026

YMC America Brings U.S.-Supplied ASME U-Stamped DAC Columns to Pharmaceutical Manufacturers

YMC America will be the First U.S.-Based Supplier of ASME U-Stamped DAC Columns. The company’s DAC columns support pharmaceutical and biopharmaceutical customers from process development through commercial production. Systems are used in laboratory, pilot, and production-scale chromatography applications, including purification processes for peptides, oligonucleotides, and small-molecule APIs.
Read More

September 10, 2026

Analytical Technologies for Therapeutic Peptides

Therapeutic peptides are an emerging class of active pharmaceutical ingredients that, together with other classes of bioproducts, have been attracting considerable attention due to their unique advantages. Their structure is based on well-defined amino acid sequences that may or may not contain structural modifications, resulting in molecules that generally range from 500 to 5000 Da. Currently, many examples can be used as medicines related to hormones, growth factors, neurotransmitters, among others.
Read More

Designed and developed by